ABIN2616351

antibody from antibodies-online

Targeting: ANKRA2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616351

Provider

antibodies-online

Product name

anti-Ankyrin Repeat, Family A (RFXANK-Like), 2 (ANKRA2) (AA 1-313) antibody

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse

Host

Rabbit

Epitope

AA 1-313

Isotype

IgG

Vial size

100 μL

Storage

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Handling

Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 acetyl Lys9 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys9 antibody for acetyl Lys9 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 acetyl Lys9 antibody at a dilution of 1 μg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: histone H3 acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys9/14/18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl- Lys14 peptide. Lane 7: acetyl-Lys18 peptide. Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide. Lane 10: unmodified Lys27 peptide.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot of Histone H3 acetyl Lys9 antibody (pAb). HeLa nuclear extract (20 μg per lane) probed with Histone H3 acetyl Lys9 antibody (1 μg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb. HeLa cells stained at 2 μg/ml with Histone H3 acetyl Lys9 antibody. Top panel: Histone H3 acetyl Lys9 antibody. Middle panel: DAPI. Bottom panel: merge.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver. ChIP DNA was sequenced on the Illumina GA II and 25 million sequence tags were mapped to identify H3K9Ac binding across the genome. The image shows a 1.5 million base pair region on chromosome 15. H3K9Ac shows promoter localization at many genes and broader binding near the Gcat gene.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

ChIP of Histone H3 acetyl Lys9 pAb. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 μg of Histone H3 acetyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.