Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PB9190 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CYP2E1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. CYP2E1 information: Molecular Weight: 56849 MW; Subcellular Localization: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease.
Drug-drug interaction of acetaminophen and roxithromycin with the cocktail of cytochrome P450 and hepatotoxicity in rats.
Hepatoprotective effect of Phellinus linteus mycelia polysaccharide (PL-N1) against acetaminophen-induced liver injury in mouse.
Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration.
Silibinin ameliorates hepatic lipid accumulation and oxidative stress in mice with non-alcoholic steatohepatitis by regulating CFLAR-JNK pathway.
Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis.
Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice.
Hepatoprotective effect of essential oils of Nepeta cataria L. on acetaminophen-induced liver dysfunction.
Chen N, Luo J, Zhou T, Shou Y, Du C, Song G, Xu L, Zhao K, Jin Y, Li C, Yu D
Biochemical pharmacology 2023 Nov 25;:115936
Biochemical pharmacology 2023 Nov 25;:115936
Drug-drug interaction of acetaminophen and roxithromycin with the cocktail of cytochrome P450 and hepatotoxicity in rats.
Liu X, Chen C, Zhang X
International journal of medical sciences 2020;17(3):414-421
International journal of medical sciences 2020;17(3):414-421
Hepatoprotective effect of Phellinus linteus mycelia polysaccharide (PL-N1) against acetaminophen-induced liver injury in mouse.
Chen C, Liu X, Qi S, C P Dias A, Yan J, Zhang X
International journal of biological macromolecules 2020 Jul 1;154:1276-1284
International journal of biological macromolecules 2020 Jul 1;154:1276-1284
Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration.
Dong S, Ji J, Hu L, Wang H
Life sciences 2019 Jun 15;227:20-29
Life sciences 2019 Jun 15;227:20-29
Silibinin ameliorates hepatic lipid accumulation and oxidative stress in mice with non-alcoholic steatohepatitis by regulating CFLAR-JNK pathway.
Liu Y, Xu W, Zhai T, You J, Chen Y
Acta pharmaceutica Sinica. B 2019 Jul;9(4):745-757
Acta pharmaceutica Sinica. B 2019 Jul;9(4):745-757
Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis.
Hu JN, Xu XY, Li W, Wang YM, Liu Y, Wang Z, Wang YP
Journal of ginseng research 2019 Jan;43(1):10-19
Journal of ginseng research 2019 Jan;43(1):10-19
Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice.
Li XX, Jiang ZH, Zhou B, Chen C, Zhang XY
Journal of physiology and biochemistry 2019 Feb;75(1):29-37
Journal of physiology and biochemistry 2019 Feb;75(1):29-37
Hepatoprotective effect of essential oils of Nepeta cataria L. on acetaminophen-induced liver dysfunction.
Tan J, Li J, Ma J, Qiao F
Bioscience reports 2019 Aug 30;39(8)
Bioscience reports 2019 Aug 30;39(8)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190). CYP2E1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image