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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-49923 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP2E1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total CYP2E1 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP2E1 in extracts from K562 cells and HepG2 cells. Samples were probed using CYP2E1 antibody (Product # PA5-49923).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CYP2E1 Polyclonal Antibody (Product # PA5-49923) and a band ~40 kDa band corresponding to CYP2E1 isoforms was observed across cell lines tested along with an isoform at ~50kDa in HeLa, MDA-MB-231, MDA-MB-231 treated with Etoposide and in Mouse and Rat Liver tissues. Membrane enriched extracts (30 µg of lysate) of Hep G2 (Lane1), Hep G2 treated with Trichostatin (1µM for 24hr ) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), MCF-7 treated with Etoposide (10µM for 16hr) (Lane 5), MCF-7 treated with Ethanol (100mM for 24hr) (Lane 6), MDA-MB-231 (Lane 7), MDA-MB-231 treated with Etoposide (10µM for 16hr) (Lane 8), and tissue extracts (30 µg) of Mouse Liver (Lane 9), Rat Liver (Lane 10) and Mouse Thymus (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CYP2E1 in HepG2 cells. Samples were probed using a CYP2E1 polyclonal antibody (Product # PA5-49923).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CYP2E1 in paraffin-embedded Human brain tissue using CYP2E1 Polyclonal Antibody (Product # PA5-49923).
