PA5-50945

antibody from Invitrogen Antibodies

Targeting: CYP2E1 CYP2E

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-50945

Provider

Invitrogen Antibodies

Product name

CYP2E1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

CYP2E1 Polyclonal Antibody detects endogenous levels of CYP2E1 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP2E1 in KB cells. Samples were probed using CYP2E1 Polyclonal Antibody (Product # PA5-50945).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CYP2E1 Polyclonal Antibody (Product # PA5-50945) and two isoforms at ~50kDa, ~40 kDa band corresponding to CYP2E1 isoforms was observed across cell lines and in Mouse and Rat Liver tissues. Membrane enriched extracts (30 µg) of HepG2 (Lane1), HepG2 treated with Trichostatin (1µM for 24hr ) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), MCF-7 treated with Etoposide (10µM for 16hr) (Lane 5), MCF-7 treated with Ethanol (100mM for 24hr) (Lane 6), MDA-MB-231 (Lane 7), MDA-MB-231 treated with Etoposide (10µM for 16hr) (Lane 8), and tissue extracts (30 µg) of Mouse Liver (Lane 9), Rat Liver (Lane 10) and Mouse Thymus (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).