PA5-52652

antibody from Invitrogen Antibodies

Targeting: CYP2E1 CYP2E

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52652

Provider

Invitrogen Antibodies

Product name

CYP2E1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: EKLHEEIDRV IGPSRIPAIK DRQEMPYMDA VVHEIQRFIT LVPSNLPHEA TRDTIFRGYL IPKGTVVVPT LDSVLYDNQE FPDPEKFKPE HFLNENGKFK YSDYFKPFST GKRVCAG

Concentration

0.08 mg/mL

References

Submitted references

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Cao YJ, Li HZ, Zhao J, Sun YM, Jin XW, Lv SQ, Luo JY, Fang XX, Wen WB, Liao JB
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2264394

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP2E1 in human liver tissue using a CYP2E1 Polyclonal Antibody (Product # PA5-52652).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP2E1 in mouse liver tissue and rat liver tissue using a CYP2E1 Polyclonal Antibody (Product # PA5-52652).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using anti-CYP2E1 Polyclonal Antibody (Product # PA5-52652) and an ~55 kDa band corresponding to CYP2E1 was observed across the cell lines and tissues tested. Few uncharacterized (*) bands were also observed in HeLa and MDA-MB-231 cells. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3) and tissue extracts (30 µg lysate) of Mouse Liver (Lane 4), Mouse Adipose (Lane 5), Mouse Heart (Lane 6), Rat Liver (Lane 7) and Rat Heart (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver using a CYP2E1 Polyclonal Antibody (Product # PA5-52652) shows moderate to strong cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver cancer (hepatocellular carcinoma) using a CYP2E1 Polyclonal Antibody (Product # PA5-52652) shows moderate cytoplasmic positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human endometrium using CYP2E1 Polyclonal Antibody (Product # PA5-52652) shows no positivity in glandular or stromal cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human tonsil using CYP2E1 Polyclonal Antibody (Product # PA5-52652) shows no positivity in germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver cancer using CYP2E1 Polyclonal Antibody (Product # PA5-52652) shows moderate cytoplasmic positivity in tumor cells.