PA5-56190

antibody from Invitrogen Antibodies

Targeting: CYP2E1 CYP2E

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56190

Provider

Invitrogen Antibodies

Product name

CYP2E1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP2E1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a CYP2E1 Polyclonal Antibody (Product # PA5-56190).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver and endometrium tissues using CYP2E1 Polyclonal Antibody (Product # PA5-56190). Corresponding CYP2E1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human endometrium, liver, liver cancer and tonsil using CYP2E1 Polyclonal Antibody (Product # PA5-56190) (A) shows similar protein distribution across tissues to an independent CYP2E1 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human endometrium using CYP2E1 Polyclonal Antibody (Product # PA5-56190) shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver using CYP2E1 Polyclonal Antibody (Product # PA5-56190) shows strong cytoplasmic positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human tonsil using CYP2E1 Polyclonal Antibody (Product # PA5-56190) shows no positivity in germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP2E1 in human liver cancer, hepatocellular carcinoma using CYP2E1 Polyclonal Antibody (Product # PA5-56190) shows moderate cytoplasmic positivity in tumor cells.