TA500735

antibody from OriGene

Targeting: CYP2E1 CYP2E

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500735

Provider

OriGene

Proper citation

OriGene Cat#TA500735, RRID:AB_11125611

Product name

Anti-CYP2E1 (Cytochrome P450 2E1) mouse monoclonal antibody, clone OTI9E6 (formerly 9E6)

Antibody type

Monoclonal

Description

Anti-CYP2E1 (Cytochrome P450 2E1) mouse monoclonal antibody, clone OTI9E6 (formerly 9E6)

Host

Mouse

Conjugate

Unconjugated

Epitope

CYP2E1

Isotype

IgG

Antibody clone number

OTI9E6

Vial size

100 µl

Concentration

0.74 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2E1 (RC213502, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1 .

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CYP2E1 mouse monoclonal antibody (TA500735) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2E1 (RC213502).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody (TA500735).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500735, Dilution 1:50)

Validation comment

IHC