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- Antigen structure
- References [11]
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- Product number
- A00672 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Cytochrome P450 2E1/CYP2E1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Drug-drug interaction of acetaminophen and roxithromycin with the cocktail of cytochrome P450 and hepatotoxicity in rats.
Hepatoprotective effect of Phellinus linteus mycelia polysaccharide (PL-N1) against acetaminophen-induced liver injury in mouse.
Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis.
Dihydrocurcumin ameliorates the lipid accumulation, oxidative stress and insulin resistance in oleic acid-induced L02 and HepG2 cells.
Effect of silibinin on CFLAR-JNK pathway in oleic acid-treated HepG2 cells.
Effect of enrofloxacin and roxarsone on CYP450s in pig.
Fructose 1, 6-diphosphate prevents alcohol-induced liver injury through inhibiting oxidative stress and promoting alcohol metabolism in mice.
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARĪ± down-regulation.
Total sesquiterpene glycosides from Loquat (Eriobotrya japonica) leaf alleviate high-fat diet induced non-alcoholic fatty liver disease through cytochrome P450 2E1 inhibition.
Hepatoprotective effect of kaempferol against alcoholic liver injury in mice.
Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression.
Liu X, Chen C, Zhang X
International journal of medical sciences 2020;17(3):414-421
International journal of medical sciences 2020;17(3):414-421
Hepatoprotective effect of Phellinus linteus mycelia polysaccharide (PL-N1) against acetaminophen-induced liver injury in mouse.
Chen C, Liu X, Qi S, C P Dias A, Yan J, Zhang X
International journal of biological macromolecules 2020 Jul 1;154:1276-1284
International journal of biological macromolecules 2020 Jul 1;154:1276-1284
Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis.
Hu JN, Xu XY, Li W, Wang YM, Liu Y, Wang Z, Wang YP
Journal of ginseng research 2019 Jan;43(1):10-19
Journal of ginseng research 2019 Jan;43(1):10-19
Dihydrocurcumin ameliorates the lipid accumulation, oxidative stress and insulin resistance in oleic acid-induced L02 and HepG2 cells.
Yu Q, Liu Y, Wu Y, Chen Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jul;103:1327-1336
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jul;103:1327-1336
Effect of silibinin on CFLAR-JNK pathway in oleic acid-treated HepG2 cells.
Liu Y, Yu Q, Chen Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Dec;108:716-723
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Dec;108:716-723
Effect of enrofloxacin and roxarsone on CYP450s in pig.
Li Y, Jiang M, Thunders M, Ai X, Qiu J
Research in veterinary science 2018 Apr;117:97-98
Research in veterinary science 2018 Apr;117:97-98
Fructose 1, 6-diphosphate prevents alcohol-induced liver injury through inhibiting oxidative stress and promoting alcohol metabolism in mice.
Li L, Wu Y, Yin F, Feng Q, Dong X, Zhang R, Yin Z, Luo L
European journal of pharmacology 2017 Nov 15;815:274-281
European journal of pharmacology 2017 Nov 15;815:274-281
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARĪ± down-regulation.
Na S, Li J, Zhang H, Li Y, Yang Z, Zhong Y, Dong G, Yang J, Yue J
Toxicology 2017 Jun 15;385:18-27
Toxicology 2017 Jun 15;385:18-27
Total sesquiterpene glycosides from Loquat (Eriobotrya japonica) leaf alleviate high-fat diet induced non-alcoholic fatty liver disease through cytochrome P450 2E1 inhibition.
Jian T, Ao X, Wu Y, Lv H, Ma L, Zhao L, Tong B, Ren B, Chen J, Li W
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jul;91:229-237
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jul;91:229-237
Hepatoprotective effect of kaempferol against alcoholic liver injury in mice.
Wang M, Sun J, Jiang Z, Xie W, Zhang X
The American journal of Chinese medicine 2015;43(2):241-54
The American journal of Chinese medicine 2015;43(2):241-54
Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression.
Chen X, Sun CK, Han GZ, Peng JY, Li Y, Liu YX, Lv YY, Liu KX, Zhou Q, Sun HJ
World journal of gastroenterology 2009 Apr 21;15(15):1829-35
World journal of gastroenterology 2009 Apr 21;15(15):1829-35
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). CYP2E1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (A00672) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
