Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-85366 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85366, RRID:AB_11015289
- Product name
- Rabbit Polyclonal Cytochrome P450 2E1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Cytochrome P450 2E1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics.
Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, Asplund A, Sjöstedt E, Lundberg E, Szigyarto CA, Skogs M, Takanen JO, Berling H, Tegel H, Mulder J, Nilsson P, Schwenk JM, Lindskog C, Danielsson F, Mardinoglu A, Sivertsson A, von Feilitzen K, Forsberg M, Zwahlen M, Olsson I, Navani S, Huss M, Nielsen J, Ponten F, Uhlén M
Molecular & cellular proteomics : MCP 2014 Feb;13(2):397-406
Molecular & cellular proteomics : MCP 2014 Feb;13(2):397-406
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85366] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium, liver, liver cancer and tonsil using Anti-CYP2E1 antibody NBP1-85366 (A) shows similar protein distribution across tissues to independent antibody NBP1-85367 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Analysis in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human tonsil shows no positivity in germinal center cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human endometrium shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver cancer, hepatocellular carcinoma shows moderate cytoplasmic positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85366] - Staining of human liver shows strong cytoplasmic positivity in tumor cells.