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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-31396 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFAF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32.
- Concentration
- 0.84 mg/mL
Submitted references SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development.
Raducu M, Fung E, Serres S, Infante P, Barberis A, Fischer R, Bristow C, Thézénas ML, Finta C, Christianson JC, Buffa FM, Kessler BM, Sibson NR, Di Marcotullio L, Toftgård R, D'Angiolella V
The EMBO journal 2016 Jul 1;35(13):1400-16
The EMBO journal 2016 Jul 1;35(13):1400-16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFAF5 Polyclonal Antibody (Product # PA5-31396). Sample (30 µg of whole cell lysate). Lane A: IMR32. 10% SDS PAGE. NDUFAF5 Polyclonal Antibody (Product # PA5-31396) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C20orf7 showing staining in the cytoplasm of SK-N-SH cells. SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a C20orf7 polyclonal antibody (Product # PA5-31396) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFAF5 Polyclonal Antibody detects C20orf7 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: C20orf7 protein stained by NDUFAF5 Polyclonal Antibody (Product # PA5-31396) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFAF5 Polyclonal Antibody detects C20orf7 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: C20orf7 protein stained by NDUFAF5 Polyclonal Antibody (Product # PA5-31396) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C20orf7 (Product # PA5-31396) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
