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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00011228-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011228-M01, RRID:AB_547618
- Product name
- Mouse Monoclonal RASSF8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RASSF8 - Ras association (RalGDS/AF-6) domain family 8
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The RASSF8 candidate tumor suppressor inhibits cell growth and regulates the Wnt and NF-kappaB signaling pathways.
Lock FE, Underhill-Day N, Dunwell T, Matallanas D, Cooper W, Hesson L, Recino A, Ward A, Pavlova T, Zabarovsky E, Grant MM, Maher ER, Chalmers AD, Kolch W, Latif F
Oncogene 2010 Jul 29;29(30):4307-16
Oncogene 2010 Jul 29;29(30):4307-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RASSF8 Antibody (2G1) [H00011228-M01] - RASSF8 monoclonal antibody (M01), clone 2G1 Western Blot analysis of RASSF8 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RASSF8 Antibody (2G1) [H00011228-M01] - RASSF8 monoclonal antibody (M01), clone 2G1 Analysis of RASSF8 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RASSF8 Antibody (2G1) [H00011228-M01] - Detection limit for recombinant GST tagged RASSF8 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RASSF8 Antibody (2G1) [H00011228-M01] - Analysis of monoclonal antibody to RASSF8 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RASSF8 Antibody (2G1) [H00011228-M01] - Analysis of RASSF8 transfected lysate using anti-RASSF8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RASSF8 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RASSF8 Antibody (2G1) [H00011228-M01] - Analysis of monoclonal antibody to RASSF8 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
