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- References [31]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 14342-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14342-1-AP, RRID:AB_2092930
- Product name
- DIAPH3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated DIAPH3 antibody (Cat. #14342-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ECM-Stiffness Mediated Persistent Fibroblast Activation Requires Integrin and Formin Dependent Chromatin Remodeling.
DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer.
Nuclear rupture in confined cell migration triggers nuclear actin polymerization to limit chromatin leakage.
The DIAPH3/RPL6 axis regulates the cGAS-STING pathway in pancreatic cancer.
Single-nucleus multiomics in brains from Hispanic individuals reveal APOE-ε4 -driven disruption of focal adhesion signaling in the presence of cerebrovascular pathology.
Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.
m1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19.
Cathepsin C Promotes Tumorigenesis in Bladder Cancer by Activating the Wnt/β-catenin Signalling Pathway.
Identification of a cisplatin resistant-based prognostic immune related gene signature in MIBC.
Formin-mediated nuclear actin at androgen receptors promotes transcription.
The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer.
mDia formins form hetero-oligomers and cooperatively maintain murine hematopoiesis.
The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.
TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.
Embedding of HIV Egress within Cortical F-Actin.
DIAPH3 is a prognostic biomarker and inhibit colorectal cancer progression through maintaining EGFR degradation.
Correction: Generation of stress fibers through myosin-driven reorganization of the actin cortex.
Overexpression of the microtubule-binding protein CLIP-170 induces a +TIP network superstructure consistent with a biomolecular condensate.
Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.
Nonredundant roles of DIAPHs in primary ciliogenesis.
Generation of stress fibers through myosin-driven reorganization of the actin cortex.
Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.
Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.
Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).
Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.
Actomyosin stress fiber subtypes have unique viscoelastic properties and roles in tension generation.
mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.
APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.
An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.
The formin FMNL3 is a cytoskeletal regulator of angiogenesis.
Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.
Packirisamy S, André O, Fan Z, Nordenfelt P, Swaminathan VS
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar 31;:e17631
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar 31;:e17631
DIAPH3 is a multifaceted prognostic biomarker that links immunotherapy response to tumor microenvironment in prostate cancer.
Chen Y, Wang P, Yang S, Jia G, Jia L, Zhu R
Discover oncology 2026 Jan 16;17(1):286
Discover oncology 2026 Jan 16;17(1):286
Nuclear rupture in confined cell migration triggers nuclear actin polymerization to limit chromatin leakage.
Kamaras C, Frank D, Wang H, Drepper F, Huesgen PF, Grosse R
The EMBO journal 2025 Nov;44(21):6112-6136
The EMBO journal 2025 Nov;44(21):6112-6136
The DIAPH3/RPL6 axis regulates the cGAS-STING pathway in pancreatic cancer.
Huang H, Lin C, Zhou C, Cui L, Rong Y
European journal of medical research 2025 Nov 29;31(1):9
European journal of medical research 2025 Nov 29;31(1):9
Single-nucleus multiomics in brains from Hispanic individuals reveal APOE-ε4 -driven disruption of focal adhesion signaling in the presence of cerebrovascular pathology.
Yilmaz E, Chen KW, Cakir EÖ, Bhattarai P, Liu M, Ciener BL, Qiao M, Lantigua R, Medrano M, Rivera D, Piriz AL, De Jager P, Bartolini F, Ma Y, Teich AF, Lee AJ, Reyes-Dumeyer D, Vardarajan BN, Mayeux R, Kizil C
medRxiv : the preprint server for health sciences 2025 May 21;
medRxiv : the preprint server for health sciences 2025 May 21;
Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.
Isogai T, Dean KM, Roudot P, Azarova EV, Bhatt K, Driscoll MK, Royer SP, Mittal N, Chang BJ, Han SJ, Fiolka R, Danuser G
iScience 2025 Jun 20;28(6):112623
iScience 2025 Jun 20;28(6):112623
m1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19.
Mi S, Hu J, Chen W, Chen J, Xu Z, Xue M
Clinical & experimental metastasis 2025 Jan 22;42(2):10
Clinical & experimental metastasis 2025 Jan 22;42(2):10
Cathepsin C Promotes Tumorigenesis in Bladder Cancer by Activating the Wnt/β-catenin Signalling Pathway.
Wang X, Jia Y, Wang D
Frontiers in bioscience (Landmark edition) 2024 Sep 23;29(9):327
Frontiers in bioscience (Landmark edition) 2024 Sep 23;29(9):327
Identification of a cisplatin resistant-based prognostic immune related gene signature in MIBC.
Wu Y, Xu Z, Fu G, Chen X, Tian J, Cai H, Jiang P, Jin B
Translational oncology 2024 Jun;44:101942
Translational oncology 2024 Jun;44:101942
Formin-mediated nuclear actin at androgen receptors promotes transcription.
Knerr J, Werner R, Schwan C, Wang H, Gebhardt P, Grötsch H, Caliebe A, Spielmann M, Holterhus PM, Grosse R, Hornig NC
Nature 2023 May;617(7961):616-622
Nature 2023 May;617(7961):616-622
The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer.
Chen X, Xie L, Qiao K, Zhu X, Ren J, Tan Y
Aging 2023 Jan 5;15(3):689-704
Aging 2023 Jan 5;15(3):689-704
mDia formins form hetero-oligomers and cooperatively maintain murine hematopoiesis.
Li Z, Su M, Xie X, Wang P, Bi H, Li E, Ren K, Dong L, Lv Z, Ma X, Liu Y, Zhao B, Peng Y, Liu J, Liu L, Yang J, Ji P, Mei Y
PLoS genetics 2023 Dec;19(12):e1011084
PLoS genetics 2023 Dec;19(12):e1011084
The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.
Becker KN, Pettee KM, Sugrue A, Reinard KA, Schroeder JL, Eisenmann KM
Cells 2022 May 5;11(9)
Cells 2022 May 5;11(9)
TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.
Huang Q, Wu D, Zhao J, Yan Z, Chen L, Guo S, Wang D, Yuan C, Wang Y, Liu X, Xing J
The EMBO journal 2022 Jun 1;41(11):e110324
The EMBO journal 2022 Jun 1;41(11):e110324
Embedding of HIV Egress within Cortical F-Actin.
Aggarwal A, Stella AO, Henry CC, Narayan K, Turville SG
Pathogens (Basel, Switzerland) 2022 Jan 3;11(1)
Pathogens (Basel, Switzerland) 2022 Jan 3;11(1)
DIAPH3 is a prognostic biomarker and inhibit colorectal cancer progression through maintaining EGFR degradation.
Huang R, Wu C, Wen J, Yu J, Zhu H, Yu J, Zou Z
Cancer medicine 2022 Dec;11(23):4688-4702
Cancer medicine 2022 Dec;11(23):4688-4702
Correction: Generation of stress fibers through myosin-driven reorganization of the actin cortex.
Lehtimäki JI, Rajakylä EK, Tojkander S, Lappalainen P
eLife 2022 Apr 20;11
eLife 2022 Apr 20;11
Overexpression of the microtubule-binding protein CLIP-170 induces a +TIP network superstructure consistent with a biomolecular condensate.
Wu YO, Bryant AT, Nelson NT, Madey AG, Fernandes GF, Goodson HV
PloS one 2021;16(12):e0260401
PloS one 2021;16(12):e0260401
Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.
Majmundar AJ, Buerger F, Forbes TA, Klämbt V, Schneider R, Deutsch K, Kitzler TM, Howden SE, Scurr M, Tan KS, Krzeminski M, Widmeier E, Braun DA, Lai E, Ullah I, Amar A, Kolb A, Eddy K, Chen CH, Salmanullah D, Dai R, Nakayama M, Ottlewski I, Kolvenbach CM, Onuchic-Whitford AC, Mao Y, Mann N, Nabhan MM, Rosen S, Forman-Kay JD, Soliman NA, Heilos A, Kain R, Aufricht C, Mane S, Lifton RP, Shril S, Little MH, Hildebrandt F
Science advances 2021 Jan;7(1)
Science advances 2021 Jan;7(1)
Nonredundant roles of DIAPHs in primary ciliogenesis.
Palander O, Lam A, Collins RF, Moraes TJ, Trimble WS
The Journal of biological chemistry 2021 Jan-Jun;296:100680
The Journal of biological chemistry 2021 Jan-Jun;296:100680
Generation of stress fibers through myosin-driven reorganization of the actin cortex.
Lehtimäki JI, Rajakylä EK, Tojkander S, Lappalainen P
eLife 2021 Jan 28;10
eLife 2021 Jan 28;10
Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.
Liu Y, Mei Y, Han X, Korobova FV, Prado MA, Yang J, Peng Z, Paulo JA, Gygi SP, Finley D, Ji P
Blood 2021 Jan 21;137(3):398-409
Blood 2021 Jan 21;137(3):398-409
Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.
Pettee KM, Becker KN, Alberts AS, Reinard KA, Schroeder JL, Eisenmann KM
Cancers 2019 Mar 20;11(3)
Cancers 2019 Mar 20;11(3)
Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).
Dvorak KM, Pettee KM, Rubinic-Minotti K, Su R, Nestor-Kalinoski A, Eisenmann KM
PloS one 2018;13(3):e0195278
PloS one 2018;13(3):e0195278
Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.
Rana MK, Aloisio FM, Choi C, Barber DL
Molecular biology of the cell 2018 Jun 15;29(12):1465-1475
Molecular biology of the cell 2018 Jun 15;29(12):1465-1475
Actomyosin stress fiber subtypes have unique viscoelastic properties and roles in tension generation.
Lee S, Kassianidou E, Kumar S
Molecular biology of the cell 2018 Aug 8;29(16):1992-2004
Molecular biology of the cell 2018 Aug 8;29(16):1992-2004
mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.
Wyse MM, Goicoechea S, Garcia-Mata R, Nestor-Kalinoski AL, Eisenmann KM
Biochemical and biophysical research communications 2017 Mar 4;484(2):255-261
Biochemical and biophysical research communications 2017 Mar 4;484(2):255-261
APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.
Castelblanco E, Zafon C, Maravall J, Gallel P, Martinez M, Capel I, Bella MR, Halperin I, Temprana J, Iglesias C, Puig-Domingo M, Robledo M, Matias-Guiu X, Mauricio D
Thyroid : official journal of the American Thyroid Association 2017 Jan;27(1):59-66
Thyroid : official journal of the American Thyroid Association 2017 Jan;27(1):59-66
An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.
Pettee KM, Dvorak KM, Nestor-Kalinoski AL, Eisenmann KM
PloS one 2014;9(2):e90371
PloS one 2014;9(2):e90371
The formin FMNL3 is a cytoskeletal regulator of angiogenesis.
Hetheridge C, Scott AN, Swain RK, Copeland JW, Higgs HN, Bicknell R, Mellor H
Journal of cell science 2012 Mar 15;125(Pt 6):1420-8
Journal of cell science 2012 Mar 15;125(Pt 6):1420-8
Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.
Stastna J, Pan X, Wang H, Kollmannsperger A, Kutscheidt S, Lohmann V, Grosse R, Fackler OT
Cell research 2012 Apr;22(4):728-45
Cell research 2012 Apr;22(4):728-45
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP(DIAPH3 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The DIAPH3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human DIAPH3. This antibody recognizes human antigen. The DIAPH3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
