PA5-25651

BHLHE41 antibody from Invitrogen Antibodies

BHLHB3, bHLHe41, DEC2, SHARP-1, SHARP1

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Flow cytometry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for PA5-25651

Antibody Data

Product number

PA5-25651

Provider

Invitrogen Antibodies

Product name

Anti-BHLH3 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a BHLH3 polyclonal antibody (Product # PA5-25651) in RD cell lysates (35 µg per lane).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using a BHLH3 polyclonal antibody (Product # PA5-25651). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a BHLH3 polyclonal antibody (Product # PA5-25651) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using a BHLH3 polyclonal antibody (Product # PA5-25651) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using a BHLH3 polyclonal antibody (Product # PA5-25651). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a BHLH3 polyclonal antibody (Product # PA5-25651) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).