PA5-25651

antibody from Invitrogen Antibodies

Targeting: BHLHE41 BHLHB3, DEC2, SHARP-1, SHARP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25651

Provider

Invitrogen Antibodies

Product name

BHLHE41 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BHLHE41 in RD cell line lysates. Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-25651). Lysates: 35 µg/lane. BHE41 protein (arrow).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BHLHE41 in Human brain lysate. Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-25651) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BHLHE41 in Mouse brain lysate. Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-25651) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using a BHLH3 polyclonal antibody (Product # PA5-25651). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a BHLH3 polyclonal antibody (Product # PA5-25651) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of BHLHE41 in MCF-7 cells. Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-25651) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). BHLH3 immunoreactivity is localized to nucleus significantly.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using a BHLH3 polyclonal antibody (Product # PA5-25651) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BHLHE41 in NCI-H460 cells (right histogram). Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-25651) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).