PA5-23065

antibody from Invitrogen Antibodies

Targeting: KDM4C GASC1, JMJD2C, KIAA0780, TDRD14C

Provider product page for PA5-23065

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Chromatin Immunoprecipitation

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [3]

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Product number: PA5-23065 - Provider product page
Provider: Invitrogen Antibodies
Product name: JMJD2C Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

KDM4C protein structure - PA5-23065 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. PITX1 is a specificity determinant for HIF-1a-dependent target gene activation. (A) U2OS and HeLa cells were transfected with control or PITX1 siRNA, prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies. (B) HeLa cells were transfected with control or PITX1 siRNA, prior to treatment with 1% O2 for the indicated periods of time. Whole cell lysates were obtained 48 hours post-transfection and analyzed by western blot using the indicated antibodies. (C) HeLa cells were transfected, processed and analyzed as in B. (D) U2OS were co-transfected with 1 mug of GFP-HIF-1beta and increasing amounts of PITX1 plasmid (0.1, 0.25 and 0.5 mug) prior to exposure to 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies. (E) MDA-MB-231-HRE cells were transfected with control and PITX1 siRNA oligonucleotides prior to treatment with 1% O2 for 24 hours. Luciferase activity was measured 48 hours post-transfection. Graph depicts mean and standard deviation of a minimum of 3 independent experiments. Student's t-test was performed to calculate p values, and levels of significance are denoted as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001. (F) MDA-MB-231 cells were transfected with control and PITX1 siRNA oligonucleotides prior to treatment to 1% O2 for 24 hours. Whole cell lys

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. PITX1 regulates JMJD2B and JMJD2C expression in a HIF-1alpha-dependent manner. (A) HeLa cells were transfected with control or PITX1 siRNA, prior to treatment with 1% O2 for 24 hours. Total mRNA was extracted and levels of JMJD2A, JMJD2B and JMJD2C mRNA were analyzed by qPCR. Graph depicts mean and standard deviation of a minimum of 3 independent experiments. Data was normalized using actin and compared to control siRNA. Student's t-test was performed to calculate p values, and levels of significance are denoted as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001. (B) HeLa cells were transfected with control or HIF-1alpha siRNA, prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies. (C) HeLa cells were transfected with control, PITX1, HIF-1a or PITX1 and HIF-1alpha siRNA, prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by western blot using the indicated antibodies. (D) HeLa cells were transfected with the indicated siRNAs oligonucleotides prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Variation in the levels of enzymes that modify histone H3 in transformed MCF10A-Er-Src cell line . A, Immunostaining to detect KDM4B demethylase and western blots detecting KDM4A, KDM4B and KDM4C histone demethylases as well as the methyl transferase SUV39H1 levels in non-transformed and transformed cells. B, Immunostaining to detect UTX/KDM6A and western blot experiments detecting UTX/KDM6A and KDM6B in non-transformed and transformed cells. C, Immunostaining to detect the methyl transferase EZH2 and western blots to detect EZH2 and SUZ12 in transformed and no-transformed cells. Acting was used as loading control in all the western blot experiments. NT=Non-transformed cells; T= Transformed cells.