PA5-141118

antibody from Invitrogen Antibodies

Targeting: ITPR2 CFAP48, IP3R2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-141118

Provider

Invitrogen Antibodies

Product name

IP3 Receptor 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ITPR2 in HepG2 cells (green). Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ITPR2 in HT-29 cells (green). Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ITPR2 in SiHa cells (green). Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ITPR2 in paraffin-embedded rat kidney tissue. Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ITPR2 in paraffin-embedded mouse liver tissue. Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ITPR2 in paraffin-embedded human breast tissue. Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ITPR2 in paraffin-embedded human colon tissue. Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ITPR2 in HepG2 cells. Samples were incubated in ITPR2 Polyclonal antibody (Product # PA5-141118) using a dilution of 1:100 (red) followed by Alexa Fluor 488-conjugated Goat anti-Rabbit IgG secondary antibody. Unlabeled control (cells without incubation with primary antibody; blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot blot analysis of ITPR2 on PVDF. Samples were incubated in ITPR2 polyclonal antibody (Product # PA5-141118) using a dilution of 1:500. Antigens (0-4 µg) were given in this test.