PA5-97677

antibody from Invitrogen Antibodies

Targeting: DUOX2 LNOX2, P138-TOX, P138(TOX), THOX2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-97677

Provider

Invitrogen Antibodies

Product name

DUOX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.84 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DUOX2 using a DUOX2 Polyclonal antibody (Product # PA5-97677) at a concentration of 2.18 µg/mL. Lane 1: Jurkat whole cell lysate. Lane 2: HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 176 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DUOX2 using a DUOX2 Polyclonal antibody (Product # PA5-97677). Positive WB detected in: 293 whole cell lysate. All lanes: DUOX2 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 176 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUOX2 in multiple sample types using a DUOX2 polyclonal antibody (Product # PA5-97677) at a dilution of 1:500. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, and Ntera-2 whole cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DUOX2 in Jurkat cells. The sample was incubated with a DUOX2 polyclonal antibody (Product # PA5-97677), followed by FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DUOX2 in Jurkat cells. The sample was incubated with a DUOX2 polyclonal antibody (Product # PA5-97677), followed by FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).