Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN187594 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19) antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant human Troy:Fc. The cysteine-rich domain of human Troy (aa 1-168) is fused to the Fc portion of human IgG1.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Giu-Li
- Vial size
- 50 μg
- Storage
- 4°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- HEK 293T cells were mock transfected (thin line) or transfected with an expression coding for the extracellular domain of human Troy (thick line). Method: Cells (5x105) were incubated on ice for 30 min in 50 μL FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of monoclonal antibody to Troy . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.