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Western blot
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-111871 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TROY (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TROY was performed in human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4). Lane 1,2: TROY (extracellular) Antibody (Product # PA5-111871) at a dilution of 1:400. Lane 3,4: TROY (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TROY was performed in new born rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4). Lane 1,2: TROY (extracellular) Antibody (Product # PA5-111871) at a dilution of 1:400. Lane 3,4: TROY (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TROY in live intact human U-87 MG cells. A) Extracellular staining of cells using TROY (extracellular) Antibody (Product # PA5-111871) (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B) Live view of the cells. C) Merge of A and B.
