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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-146-C050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-146-C050, RRID:AB_2052745
- Product name
- Troy (human) monoclonal antibody (Giu.Li)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Giu.Li
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
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- Enzo Life Sciences (provider)
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- Experimental details
- HEK 293T cells were mock transfected (thin line) or transfected with an expression coding for the extracellular domain of human Troy (thick line). Method: Cells (5x10E5) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of monoclonal antibody to Troy (Giu.Li). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.
