PA1-29792

antibody from Invitrogen Antibodies

Targeting: POR CYPOR, FLJ26468

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-29792

Provider

Invitrogen Antibodies

Product name

Cytochrome P450 Reductase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

Suggested positive controls for this product are rat liver, NADPH, cytochrome, P450 and reductase protein.

Reactivity

Human, Mouse, Rat, Canine, Guinea Pig, Hamster, Porcine, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of NADPH--cytochrome P450 reductase was achieved by transfecting HeLa with NADPH--cytochrome P450 reductase specific siRNAs (Silencer® select Product # S10839, S10838). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the NADPH--cytochrome P450 reductase knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with Cytochrome P450 Reductase Polyclonal Antibody (Product # PA1-29792 at a concentration of 0.5 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NADPH--cytochrome P450 reductase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-Cytochrome P450 Reductase Polyclonal Antibody (Product # PA1-29792) and a 77 kDa band corresponding to NADPH--cytochrome P450 reductase was observed across the cell lines teste. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and Hep G2 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (at a concentration of 0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).