Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6340 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal POR/Cytochrome P450 Reductase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects recombinant human POR/Cytochrome P450 Reductase in direct ELISAs and human, mouse, and rat POR/Cytochrome P450 Reductase in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat POR/Cytochrome P450 Reductase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.