MA5-34675

antibody from Invitrogen Antibodies

Targeting: POR CYPOR, FLJ26468

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34675

Provider

Invitrogen Antibodies

Product name

Cytochrome P450 Reductase Recombinant Rabbit Monoclonal Antibody (JB54-31)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SK-Br-3, A549, mouse lung tissue lysate, rat brain tissue, human lung cancer tissue, human liver tissue, mouse testis tissue, HepG2.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB54-31

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cytochrome P450 Reductase in SK-Br-3 (1), A549 (2) and mouse lung (3) lysate. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cytochrome P450 Reductase in SK-Br-3 (1), A549 (2) and mouse lung (3) lysate. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 Reductase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 Reductase in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytochrome P450 Reductase in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 Reductase in paraffin-embedded rat brain tissue. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 Reductase in paraffin-embedded mouse testis tissue. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 Reductase in paraffin-embedded human lung cancer tissue. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cytochrome P450 Reductase in paraffin-embedded human liver tissue. Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Cytochrome P450 Reductase in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Cytochrome P450 Reductase monoclonal antibody (Product # MA5-34675) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.