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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27326 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome P450 Reductase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse lung. Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (84%), Dog (91%), Pig (92%), Rabbit (92%), Chicken (85%), Rhesus Monkey (99%), Bovine (92%), Guinea pig (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The hypoxia-activated prodrug evofosfamide in combination with multiple regimens of radiotherapy.
Nytko KJ, Grgic I, Bender S, Ott J, Guckenberger M, Riesterer O, Pruschy M
Oncotarget 2017 Apr 4;8(14):23702-23712
Oncotarget 2017 Apr 4;8(14):23702-23712
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cytochrome P450 Reductase using 30 µg of HeLa S3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Cytochrome P450 Reductase polyclonal antibody (Product # PA5-27326) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytochrome P450 Reductase Polyclonal Antibody detects POR protein by western blot analysis. A. 50 µg mouse lung lysate/extract.7.5% SDS-PAGE. Cytochrome P450 Reductase Polyclonal Antibody (Product # PA5-27326) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytochrome P450 Reductase in methanol-fixed HeLa cells using a Cytochrome P450 Reductase polyclonal antibody (Product # PA5-27326) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POR antibody detects POR protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. POR antibody (Product # PA5-27326) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Differential POR- and PLGF-levels in A549 and UT-SCC-14 tumors (A) Protein levels of cytochrome P450 oxidoreductase (POR) in A549 and UT-SCC-14 cells incubated under normoxia (21% O 2 ) and hypoxia (0.2% O 2 , 24 hours) as determined by western blotting. (B) Staining of A549 (left) and UT-SCC-14 (right)-derived tumor xenografts sections with anti-POR antibodies. (C) Levels of secreted PLGF in A549 and UT-SCC-14 conditioned medium in normoxia (21% O 2 ) and hypoxia (0.2% O 2 , 24 hours) as determined using Bio-plex assay. Data are shown as fold induction over non-treated normoxic samples in three independent experiments, error bars represent SEM.
