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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27327 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome P450 Reductase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLaS3, HepG2. Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (84%), Dog (97%), Pig (96%), Rabbit (96%), Chicken (90%), Rhesus Monkey (100%), Bovine (94%), Guinea pig (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytochrome P450 Reductase Polyclonal Antibody (Product # PA5-27327). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. Cytochrome P450 Reductase Polyclonal Antibody (Product # PA5-27327) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytochrome P450 Reductase Polyclonal Antibody detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Cytochrome P450 Reductase Polyclonal Antibody (Product # PA5-27327) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
