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Western blot
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- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2408 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2408, RRID:AB_1581135
- Product name
- SUMO2/3 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SUMO2/3.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 uL
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity.
Degerny C, Monte D, Beaudoin C, Jaffray E, Portois L, Hay RT, de Launoit Y, Baert JL
The Journal of biological chemistry 2005 Jul 1;280(26):24330-8
The Journal of biological chemistry 2005 Jul 1;280(26):24330-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SUMO2/3 polyclonal antibody (Cat # PAB2408) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) . Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels : cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels : cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO1 polyclonal antibody (Cat # PAB2402) . Bottom panels : cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO2/3 polyclonal antibody (Cat # PAB2408) . (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the SUMO2/3 polyclonal antibody (Cat # PAB2408) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
