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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- F42562 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SUMO2/3 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific SUMO2/3 antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry/ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the SUMO2/3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SUMO2/3 antibody western blot analysis in (1) 293 cell line and (2) rat liver tissue lysate. Observed molecular weight: 12-15 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cell stained with SUMO2/3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37oC). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). immunoreactivity is localized to the nucleus.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human bladder section using SUMO2/3 antibody; Ab was diluted at 1:100.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the SUMO2/3 antibody
