PAB1859

antibody from Abnova Corporation

Targeting: SUMO3 SMT3A, SMT3H1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB1859

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB1859, RRID:AB_1581137

Product name

SUMO3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against full length recombinant SUMO3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription.

Ling Y, Sankpal UT, Robertson AK, McNally JG, Karpova T, Robertson KD
Nucleic acids research 2004;32(2):598-610

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Characterization of the localization and proteolytic activity of the SUMO-specific protease, SENP1.

Bailey D, O'Hare P
The Journal of biological chemistry 2004 Jan 2;279(1):692-703

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SUMO promotes HDAC-mediated transcriptional repression.

Yang SH, Sharrocks AD
Molecular cell 2004 Feb 27;13(4):611-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The SUMO3 polyclonal antibody (Cat # PAB1859) is used in Western blot to detect SUMO3 in GST-Sumo3 (lane 1) and 293 cell lysate (lane 2) .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SUMO3 polyclonal antibody (Cat # PAB1859), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)