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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 703741 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TDRD3 Recombinant Rabbit Monoclonal Antibody (6H18L56)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 6H18L56
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous TDRD3 using Anti-TDRD3 Recombinant Rabbit Monoclonal Antibody (Product # 703741, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TDRD3 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic and nuclear localization of TDRD3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay for endogenous TDRD3 protein using TDRD3 Antibody: ChIP was performed with the MAGnify ChIP System kit (Product # 49-2024) using TDRD3 Recombinant Rabbit Monoclonal Antibody (Product # 703741, 5 µg) on sheared chromatin from 2 million SH-SY5Y cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to the promoter regions of DNAJC9, NANOG, NRAS, gene bodies of TRIAP1, EGR1 and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
