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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-46821 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46821, RRID:AB_10009884
- Product name
- Rabbit Polyclonal TDRD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TDRD3 Antibody [NBP1-46821] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TDRD3 antibody NBP1-46821 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TDRD3 was also immunoprecipitated by rabbit anti-TDRD3 antibody NBP1-46820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821] - FFPE section of human lung carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TDRD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
