MA5-48340

antibody from Invitrogen Antibodies

Targeting: TDRD3 FLJ21007

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-48340

Provider

Invitrogen Antibodies

Product name

TDRD3 Chimeric Recombinant Rabbit Monoclonal Antibody (RAB-C419)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Specificity: This antibody recognizes TDRD3 (Tudor domain-containing protein 3). It binds to a folded domain, amino acids 1-455. TDRD3 is a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

RAB-C419

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of TDRD3 in HeLa cells using TDRD3 Chimeric Monoclonal Antibody (Product # MA5-48340). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton and stained with TDRD3 Chimeric Monoclonal Antibody at a dilution of 1:100 for 1h followed by Alexa Fluor 488 secondary antibody (1:1,500 dilution), showing nucleoli, nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom TDRD3 Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Rabbit IgG Chimeric Monoclonal Antibody (Product # MA5-47825) followed by staining with Alexa Fluor 488 secondary antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TDRD3 in HeLa cells using TDRD3 Chimeric Monoclonal Antibody (Product # MA5-48340). Cells were fixed with paraformaldehyde, permeabilized with 0.5% triton and stained with Rabbit IgG Isotype Control (Product # MA5-47825) (black line) or TDRD3 Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AF488 antibody at a dilution of 1:1,000 and cells analyzed using a flow-cytometer.