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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AMM86065 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- FGFR1OP2 Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity Purification
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Overlay histogram showing U-2OS cells stained with AM8701b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8701b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
