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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AFR-053-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLRT3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1-2. Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200). 3-4. Anti-FLRT3 (extracellular) Antibody , preincubated with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of FLRT3 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G) and in the hilus (H, arrows). B. Pre-incubation of the Antibody with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of FLRT3 in mouse cerebellum. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. FLRT3 immunoreactivity (green) appears in the purkinje cell layer (P, arrows). B. Pre-incubation of the Antibody with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of FLRT3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-APC. (green) Cells + Anti-FLRT3 (extracellular) Antibody (#AFR-053), (5μg) + goat- Anti-rabbit-APC.
