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- Antibody Data
- Antigen structure
- References [20]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB2125-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LYVE-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BHC0- Isotype
- IgG
- Antibody clone number
- 223322
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes.
T Cells in Nonlymphoid Tissues Give Rise to Lymph-Node-Resident Memory T Cells.
BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer.
Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.
Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib.
Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration.
Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways.
Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle.
Fusing VE-cadherin to α-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
A micro-sterile inflammation array as an adjuvant for influenza vaccines.
A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.
FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus.
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress.
Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.
CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells.
Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor.
Veerman K, Tardiveau C, Martins F, Coudert J, Girard JP
Cell reports 2019 Mar 12;26(11):3116-3131.e5
Cell reports 2019 Mar 12;26(11):3116-3131.e5
T Cells in Nonlymphoid Tissues Give Rise to Lymph-Node-Resident Memory T Cells.
Beura LK, Wijeyesinghe S, Thompson EA, Macchietto MG, Rosato PC, Pierson MJ, Schenkel JM, Mitchell JS, Vezys V, Fife BT, Shen S, Masopust D
Immunity 2018 Feb 20;48(2):327-338.e5
Immunity 2018 Feb 20;48(2):327-338.e5
BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer.
Ouarné M, Bouvard C, Boneva G, Mallet C, Ribeiro J, Desroches-Castan A, Soleilhac E, Tillet E, Peyruchaud O, Bailly S
Journal of experimental & clinical cancer research : CR 2018 Aug 30;37(1):209
Journal of experimental & clinical cancer research : CR 2018 Aug 30;37(1):209
Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.
Frye M, Taddei A, Dierkes C, Martinez-Corral I, Fielden M, Ortsäter H, Kazenwadel J, Calado DP, Ostergaard P, Salminen M, He L, Harvey NL, Kiefer F, Mäkinen T
Nature communications 2018 Apr 17;9(1):1511
Nature communications 2018 Apr 17;9(1):1511
Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
Johnson LA, Banerji S, Lawrance W, Gileadi U, Prota G, Holder KA, Roshorm YM, Hanke T, Cerundolo V, Gale NW, Jackson DG
Nature immunology 2017 Jul;18(7):762-770
Nature immunology 2017 Jul;18(7):762-770
A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib.
Sun T, Yang L, Kaur H, Pestel J, Looso M, Nolte H, Krasel C, Heil D, Krishnan RK, Santoni MJ, Borg JP, Bünemann M, Offermanns S, Swiercz JM, Worzfeld T
The Journal of cell biology 2017 Jan 2;216(1):199-215
The Journal of cell biology 2017 Jan 2;216(1):199-215
Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration.
Lo TH, Silveira PA, Fromm PD, Verma ND, Vu PA, Kupresanin F, Adam R, Kato M, Cogger VC, Clark GJ, Hart DN
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 1;197(3):885-98
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 1;197(3):885-98
Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways.
Wu M, Du Y, Liu Y, He Y, Yang C, Wang W, Gao F
PloS one 2014;9(3):e92857
PloS one 2014;9(3):e92857
Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle.
Thorek DL, Ulmert D, Diop NF, Lupu ME, Doran MG, Huang R, Abou DS, Larson SM, Grimm J
Nature communications 2014;5:3097
Nature communications 2014;5:3097
Fusing VE-cadherin to α-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
Dartsch N, Schulte D, Hägerling R, Kiefer F, Vestweber D
Molecular and cellular biology 2014 May;34(9):1634-48
Molecular and cellular biology 2014 May;34(9):1634-48
A micro-sterile inflammation array as an adjuvant for influenza vaccines.
Wang J, Shah D, Chen X, Anderson RR, Wu MX
Nature communications 2014 Jul 18;5:4447
Nature communications 2014 Jul 18;5:4447
A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.
Reichardt P, Patzak I, Jones K, Etemire E, Gunzer M, Hogg N
The EMBO journal 2013 Mar 20;32(6):829-43
The EMBO journal 2013 Mar 20;32(6):829-43
FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus.
Zhi L, Kim P, Thompson BD, Pitsillides C, Bankovich AJ, Yun SH, Lin CP, Cyster JG, Wu MX
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2244-51
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2244-51
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64
The Journal of clinical investigation 2009 Oct;119(10):2954-64
Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG
Nature immunology 2009 Jan;10(1):58-65
Nature immunology 2009 Jan;10(1):58-65
Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):7137-42
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):7137-42
S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress.
Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG
Immunity 2008 Jan;28(1):122-33
Immunity 2008 Jan;28(1):122-33
Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.
Phan TG, Grigorova I, Okada T, Cyster JG
Nature immunology 2007 Sep;8(9):992-1000
Nature immunology 2007 Sep;8(9):992-1000
CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells.
Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y
The American journal of pathology 2007 Oct;171(4):1249-57
The American journal of pathology 2007 Oct;171(4):1249-57
Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor.
Bäckhed F, Crawford PA, O'Donnell D, Gordon JI
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 9;104(2):606-11
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 9;104(2):606-11
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse LYVE-1 by Western Blot. Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LYVE-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
