PA1-16635

antibody from Invitrogen Antibodies

Targeting: LYVE1 LYVE-1, XLKD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16635

Provider

Invitrogen Antibodies

Product name

LYVE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: mouse spleen lysate or protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Pilot study supporting the existence of novel lymphatic channels within the canine anterior uveal tract using Lyve-1 and CD31.

Dubin A, Freeman KS, Charles J, Ammar DA, Ehrhart EJ
Veterinary ophthalmology 2021 Jul;24(4):354-360

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Systematic Evaluation of Chemically Distinct Tissue Optical Clearing Techniques in Murine Lymph Nodes.

Matryba P, Sosnowska A, Wolny A, Bozycki L, Greig A, Grzybowski J, Stefaniuk M, Nowis D, Gołąb J
Journal of immunology (Baltimore, Md. : 1950) 2020 Mar 1;204(5):1395-1407

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Prometastatic Molecular Profiles in Breast Tumors From Socially Isolated Women.

Bower JE, Shiao SL, Sullivan P, Lamkin DM, Atienza R, Mercado F, Arevalo J, Asher A, Ganz PA, Cole SW
JNCI cancer spectrum 2018 Jul;2(3):pky029

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of LYVE-1 in mouse spleen lysate using Product # PA1-16635. ECL detection overnight.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LYVE1 in human stomach, lymph node and placenta. Samples were incubated in LYVE1 polyclonal antibody (Product # PA1-16635) using a dilution of 1.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LYVE1 in endothelial cells of human lung blood vessels. Samples were incubated in LYVE1 polyclonal antibody (Product # PA1-16635). Note presence of RBCs within vessel lumen.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LYVE1 in endothelial cells of human bladder vasculature. Samples were incubated in LYVE1 polyclonal antibody (Product # PA1-16635).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LYVE1 in endothelial cells of human lung blood vessels. Samples were incubated in LYVE1 polyclonal antibody (Product # PA1-16635).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of LYVE1 in A549 cells. Samples were incubated in LYVE1 polyclonal antibody (Product # PA1-16635) using a dilution of 1:400 followed by a Alexa Fluor 488 secondary (shown in green). Unstained cells (shown in red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Analyses of lymphovascular invasion in primary breast tumors indicated increased intratumoral lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) microvessel density in tumors from socially isolated women. A) Representative images of LYVE-1-stained sections from patients reporting high vs low social isolation. B) Box plots of predicted scores for LYVE-1 microvessel density in the high- and low-social isolation groups. Each box indicates the interquartile range, and the middle line indicates the median for that group. LYVE-1 = lymphatic vessel endothelial hyaluronan receptor 1.