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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF2089 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LYVE-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant mouse LYVE-1 and less than 1% cross-reactivity with recombinant human CD44 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Y7- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Epithelial Membrane Protein-2 (EMP2) Antibody Blockade Reduces Corneal Neovascularization in an In Vivo Model.
Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques.
Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer.
Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis.
The effect of neoadjuvant chemotherapy on the correlation of tumor-associated macrophages with CD31 and LYVE-1.
Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease.
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations.
TGF-β1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT).
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Adrenomedullin blockade suppresses growth of human hormone-independent prostate tumor xenograft in mice.
An in vivo platform for tumor biomarker assessment.
Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Sun MM, Chan AM, Law SM, Duarte S, Diaz-Aguilar D, Wadehra M, Gordon LK
Investigative ophthalmology & visual science 2019 Jan 2;60(1):245-254
Investigative ophthalmology & visual science 2019 Jan 2;60(1):245-254
Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques.
Ganchua SKC, Cadena AM, Maiello P, Gideon HP, Myers AJ, Junecko BF, Klein EC, Lin PL, Mattila JT, Flynn JL
PLoS pathogens 2018 Nov;14(11):e1007337
PLoS pathogens 2018 Nov;14(11):e1007337
Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer.
Yuge R, Kitadai Y, Takigawa H, Naito T, Oue N, Yasui W, Tanaka S, Chayama K
Translational oncology 2018 Jun;11(3):575-584
Translational oncology 2018 Jun;11(3):575-584
Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis.
Cochain C, Vafadarnejad E, Arampatzi P, Pelisek J, Winkels H, Ley K, Wolf D, Saliba AE, Zernecke A
Circulation research 2018 Jun 8;122(12):1661-1674
Circulation research 2018 Jun 8;122(12):1661-1674
The effect of neoadjuvant chemotherapy on the correlation of tumor-associated macrophages with CD31 and LYVE-1.
Mitrofanova I, Zavyalova M, Riabov V, Cherdyntseva N, Kzhyshkowska J
Immunobiology 2018 Jun - Jul;223(6-7):449-459
Immunobiology 2018 Jun - Jul;223(6-7):449-459
Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease.
Wen YR, Yang JH, Wang X, Yao ZB
Neural regeneration research 2018 Apr;13(4):709-716
Neural regeneration research 2018 Apr;13(4):709-716
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Volk-Draper LD, Hall KL, Wilber AC, Ran S
PloS one 2017;12(6):e0179257
PloS one 2017;12(6):e0179257
VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations.
Hägerling R, Drees D, Scherzinger A, Dierkes C, Martin-Almedina S, Butz S, Gordon K, Schäfers M, Hinrichs K, Ostergaard P, Vestweber D, Goerge T, Mansour S, Jiang X, Mortimer PS, Kiefer F
JCI insight 2017 Aug 17;2(16)
JCI insight 2017 Aug 17;2(16)
TGF-β1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT).
Scherer SD, Bauer J, Schmaus A, Neumaier C, Herskind C, Veldwijk MR, Wenz F, Sleeman JP
PloS one 2016;11(9):e0162221
PloS one 2016;11(9):e0162221
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
Adrenomedullin blockade suppresses growth of human hormone-independent prostate tumor xenograft in mice.
Berenguer-Daizé C, Boudouresque F, Bastide C, Tounsi A, Benyahia Z, Acunzo J, Dussault N, Delfino C, Baeza N, Daniel L, Cayol M, Rossi D, El Battari A, Bertin D, Mabrouk K, Martin PM, Ouafik L
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Nov 15;19(22):6138-50
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Nov 15;19(22):6138-50
An in vivo platform for tumor biomarker assessment.
Servais EL, Suzuki K, Colovos C, Rodriguez L, Sima C, Fleisher M, Rusch VW, Sadelain M, Adusumilli PS
PloS one 2011;6(10):e26722
PloS one 2011;6(10):e26722
Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
Kivelä R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
American journal of physiology. Heart and circulatory physiology 2007 Oct;293(4):H2573-9
American journal of physiology. Heart and circulatory physiology 2007 Oct;293(4):H2573-9
Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
The Journal of clinical investigation 2007 Nov;117(11):3369-82
The Journal of clinical investigation 2007 Nov;117(11):3369-82
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis and rheumatism 2007 Jun;56(6):1817-26
Arthritis and rheumatism 2007 Jun;56(6):1817-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LYVE-1 by Western Blot. Western blot shows lysates of human liver and spleen tissue. PVDF membrane was probed with 0.25-1 µg/mL of Goat Anti-Human LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LYVE-1 in Human Tonsil. LYVE-1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
