CABT-BL451

antibody from Creative Diagnostics

Targeting: BORA C13orf34, FLJ22624

Western blot (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

CABT-BL451

Provider

Creative Diagnostics

Product name

Rabbit Anti-Human Bora monoclonal antibody, clone 20I3M2

Antibody type

Monoclonal

Description

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

20I3M2

Concentration

0.5 mg/ml

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.