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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM09375PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09375PU-S, RRID:AB_10755329
- Product name
- anti TUBB2B
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human TUBB2b (1-445aa) purified from E. coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT5B3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis: Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human TUBB2b antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
