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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA1-91112 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM5 Monoclonal Antibody (CRCT5.1 (A2.7A3))
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CRCT5.1 (A2.7A3)
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Gene regulation profile reveals consistent anticancer properties of progesterone in hormone-independent breast cancer cells transfected with progesterone receptor.
Leo JC, Wang SM, Guo CH, Aw SE, Zhao Y, Li JM, Hui KM, Lin VC
International journal of cancer 2005 Nov 20;117(4):561-8
International journal of cancer 2005 Nov 20;117(4):561-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MCM5 Monoclonal Antibody (CRCT5.1 (A2.7A3))(Product # MA1-91112) and a 82kDa band corresponding to MCM5 was observed across cell lines and tissues tested with an uncharacterized band (*) at ~25kDa. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), PC-3 (Lane 2), U-2OS (Lane 3), SK-BR-3 (Lane 4), IMR-32 (Lane 5), Jurkat (Lane 6), Mouse Embryo (Lane 7), Mouse Placenta (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MCM5 in human tonsil tissue using MCM5 Monoclonal Antibody (CRCT5.1 (A2.7A3)) (Product # MA1-91112).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MCM5 in human tonsil tissue using MCM5 Monoclonal Antibody (CRCT5.1 (A2.7A3)) (Product # MA1-91112).
