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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52638PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52638PU-N, RRID:AB_11148523
- Product name
- anti MCM5 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 705~734 amino acids from the C-terminal region of human MCM5
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of MCM5 Antibody (C-term) Cat.-No AP52638PU-N pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. MCM5 (arrow) was detected using the purified Pab (1:60 dilution).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma reacted with MCM5 Antibody (C-term) Cat.-No AP52638PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using MCM5 Antibody (C-term) Cat.-No AP52638PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
