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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-29913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Mouse (95%), Xenopus laevis (88%), Chicken (87%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCM5 Polyclonal Antibody (Product # PA5-29913). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. MCM5 Polyclonal Antibody . MCM5 Polyclonal Antibody (Product # PA5-29913) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCM5 Polyclonal Antibody detects MCM5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM5 protein stained by MCM5 Polyclonal Antibody (Product # PA5-29913) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using MCM5 (Product # PA5-29913) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous MCM5 protein using Anti-MCM5 Antibody: ChIP was performed using Anti-MCM5 Rabbit Polyclonal Antibody (Product # PA5-29913) 5 µg, on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to promoter of MYC, MCM4 transcriptional start site and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
