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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- 14413-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14413-1-AP, RRID:AB_10858916
- Product name
- PIBF1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PIBF1 antibody (Cat. #14413-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A ciliopathy complex builds distal appendages to initiate ciliogenesis.
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kumar D, Rains A, Herranz-Pérez V, Lu Q, Shi X, Swaney DL, Stevenson E, Krogan NJ, Huang B, Westlake C, Garcia-Verdugo JM, Yoder BK, Reiter JF
The Journal of cell biology 2021 Sep 6;220(9)
The Journal of cell biology 2021 Sep 6;220(9)
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
Gurkaslar HK, Culfa E, Arslanhan MD, Lince-Faria M, Firat-Karalar EN
Cell reports 2020 May 12;31(6):107630
Cell reports 2020 May 12;31(6):107630
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4
eLife 2015 Aug 22;4
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP(PIBF1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PIBF1 antibody 14413-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-PIBF1(14413-1-AP for IP and Detection).
- Sample type
- cell line
