Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 20760002-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STOML3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product is specific for Human STOML3.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: STOML3 Antibody [20760002] - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STOML3 Antibody [20760002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: STOML3 Antibody [20760002]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: STOML3 Antibody [20760002] - Cells in lymphoid follicles were negative. Endothelial cells were distinctly stained. Strong membranous positivity was observed in fallopian tube, hepatocytes and gallbladder. Among malignancies, most tissues displayed moderate cytoplasmic staining. Distinct additional membranous positivity was observed in several adenocarcinomas of colon, breast, cervix, liver, ovary and also in melanomas. Image and statement courtesy of the Human Protein Atlas (HPA).