LS-B11401

antibody from LSBio

Targeting: ARHGEF18 KIAA0521, MGC15913, P114-RhoGEF, p114RhoGEF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B11401

Provider

LSBio

Product name

IHC-plus™ ARHGEF18 Antibody LS-B11401

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human, Canine

Host

Rabbit

Isotype

IgG

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of Rho guanine nucleotide exchange factor 18 antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 129 kDa. Observed band size: 30 kDa Additional bands at: 60 kDa. We are unsure as to the identity of this extra band. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: Rho guanine nucleotide exchange factor 18 antibody at 2µg/ml Lane 1: EC109whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 131, 115 kDa Observed band size: 30, 60 kDa