PA5-21429

antibody from Invitrogen Antibodies

Targeting: ARHGEF18 KIAA0521, MGC15913, P114-RhoGEF, p114RhoGEF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21429

Provider

Invitrogen Antibodies

Product name

ARHGEF18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2. Predicted reactivity: Mouse (89%), Rat (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

GPCR-independent activation of G proteins promotes apical cell constriction in vivo.

Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M
The Journal of cell biology 2019 May 6;218(5):1743-1763

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ARHGEF18 in methanol-fixed A431 cells using an ARHGEF18 polyclonal antibody (Product # PA5-21429) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody (Product # PA5-21429) at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody (Product # PA5-21429) at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody (Product # PA5-21429) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ARHGEF18 was performed in paraffin-embedded rat spleen tissue using ARHGEF18 Polyclonal Antibody (Product # PA5-21429) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ARHGEF18 was performed in paraffin-embedded mouse thymus gland tissue using ARHGEF18 Polyclonal Antibody (Product # PA5-21429) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Both the PBM and GBA motif of DAPLE are required to promote apical cell constriction. (A) Quantification of the relative apical area of DAPLE-transfected cells compared with neighboring, untransfected cells shows that hDAPLE ΔPBM and hDAPLE GBA* mutants fail to promote apical constriction in MDCK (left) or EpH4 (right) cells. Results are presented as box-and-whiskers plots (error bars indicate minimum to maximum range) of n = 4-9 independent experiments per condition. *, P < 0.05; **, P < 0.01 using the Mann-Whitney U test. (B) Confocal fluorescence microscopy pictures of MDCK cells sparsely expressing the indicated MYC-hDAPLE constructs and costained for MYC (magenta) and ZO-1 (green) show that hDAPLE GBA* mutant is enriched at apical junctions like WT, while hDAPLE ΔPBM mutant is not. Scale bars, 10 µm. (C) Diagram depicting the different functions of the PBM and the GBA motif in DAPLE-induced apical cell constriction. A possible effector pathway to promote apical cell constriction though G protein activation is shown on the right, along with the treatments used in D to test it (green). (D) Box-and-whiskers plots (error bars indicate minimum to maximum range) for the quantification of relative apical area show that DAPLE-mediated apical constriction requires the activity of myosin (inhibited by blebbistatin), ROCK (inhibited by Y-27632), free Gßγ (inhibited by Gallein, but not its inactive analogue, fluorescein) and p114RhoGEF (inhibited by siRNA). n = 4 independent experim