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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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- Product number
- PA5-27876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HCT116, KLF5-transfected 293T. Predicted reactivity: Mouse (89%), Rat (90%), Pig (96%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Epigenetic reader BRD4 supports mycobacterial pathogenesis by co-modulating host lipophagy and angiogenesis.
Mukherjee T, Bhatt B, Prakhar P, Lohia GK, Rajmani RS, Balaji KN
Autophagy 2022 Feb;18(2):391-408
Autophagy 2022 Feb;18(2):391-408
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLF5 using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a KLF5 polyclonal antibody (Product # PA5-27876) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KLF5 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF5 Polyclonal Antibody (Product # PA5-27876) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KLF5 Polyclonal Antibody (Product # PA5-27876). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KLF5 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF5 Polyclonal Antibody (Product # PA5-27876) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KLF5 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF5 Polyclonal Antibody (Product # PA5-27876) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KLF5 was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF5 Polyclonal Antibody (Product # PA5-27876) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KLF5 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF5 Polyclonal Antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. KLF5 Polyclonal Antibody (Product # PA5-27876) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
