PA5-27876

antibody from Invitrogen Antibodies

Targeting: KLF5 BTEB2, CKLF, IKLF

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-27876 - Provider product page
Provider: Invitrogen Antibodies
Product name: KLF5 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A549, HCT116, KLF5-transfected 293T. Predicted reactivity: Mouse (89%), Rat (90%), Pig (96%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

KLF5 protein structure - PA5-27876 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of KLF5 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KLF5 Polyclonal Antibody detects KLF5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 stained by KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of KLF5 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: KLF5 Polyclonal Antibody (Product # PA5-27876) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.