17250-1-AP

antibody from Proteintech Group

Targeting: RB1CC1 ATG17, Cc1, DRAGOU14, FIP200, KIAA0203, PPP1R131

Provider product page for 17250-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 17250-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17250-1-AP, RRID:AB_10666428
Product name: RB1CC1 antibody
Antibody type: Polyclonal
Description: KD/KO validated RB1CC1 antibody (Cat. #17250-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RB1CC1 protein structure - 17250-1-AP shown in red.

Submitted references A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Li S, Yan R, Xu J, Zhao S, Ma X, Sun Q, Zhang M, Li Y, Liu JG, Chen L, Li S, Xu K, Ge L
Cell research 2021 Sep 24;

SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores.

Lee JE, Kim N, Jung M, Mun JY, Yoo JY
Autophagy 2021 Oct 31;:1-16

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes.

Kumar S, Javed R, Mudd M, Pallikkuth S, Lidke KA, Jain A, Tangavelou K, Gudmundsson SR, Ye C, Rusten TE, Anonsen JH, Lystad AH, Claude-Taupin A, Simonsen A, Salemi M, Phinney B, Li J, Guo LW, Bradfute SB, Timmins GS, Eskelinen EL, Deretic V
Cell 2021 Nov 24;184(24):5950-5969.e22

NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA.

Yamamoto Y, Chino H, Tsukamoto S, Ode KL, Ueda HR, Mizushima N
Nature communications 2021 Jun 2;12(1):3292

LUBAC and OTULIN regulate autophagy initiation and maturation by mediating the linear ubiquitination and the stabilization of ATG13.

Chu Y, Kang Y, Yan C, Yang C, Zhang T, Huo H, Liu Y
Autophagy 2021 Jul;17(7):1684-1699

FIP200 controls the TBK1 activation threshold at SQSTM1/p62-positive condensates.

Schlütermann D, Berleth N, Deitersen J, Wallot-Hieke N, Friesen O, Wu W, Stuhldreier F, Sun Y, Berning L, Friedrich A, Mendiburo MJ, Peter C, Wiek C, Hanenberg H, Stefanski A, Stühler K, Stork B
Scientific reports 2021 Jul 5;11(1):13863

Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy.

Mercer TJ, Ohashi Y, Boeing S, Jefferies HBJ, De Tito S, Flynn H, Tremel S, Zhang W, Wirth M, Frith D, Snijders AP, Williams RL, Tooze SA
The EMBO journal 2021 Jul 15;40(14):e105985

The Autophagy-Initiating Protein Kinase ULK1 Phosphorylates Human Cytomegalovirus Tegument Protein pp28 and Regulates Efficient Virus Release.

König P, Svrlanska A, Read C, Feichtinger S, Stamminger T
Journal of virology 2021 Feb 24;95(6)

ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.

Miao G, Zhao H, Li Y, Ji M, Chen Y, Shi Y, Bi Y, Wang P, Zhang H
Developmental cell 2021 Feb 22;56(4):427-442.e5

Phase-separated protein droplets of amyotrophic lateral sclerosis-associated p62/SQSTM1 mutants show reduced inner fluidity.

Faruk MO, Ichimura Y, Kageyama S, Komatsu-Hirota S, El-Gowily AH, Sou YS, Koike M, Noda NN, Komatsu M
The Journal of biological chemistry 2021 Dec;297(6):101405

Inhibiting autophagy increases the efficacy of low-dose photodynamic therapy.

Weisheit S, Wegner CS, Ailte I, Radulovic M, Weyergang A, Selbo PK, Brech A
Biochemical pharmacology 2021 Dec;194:114837

PINK1 drives production of mtDNA-containing extracellular vesicles to promote invasiveness.

Rabas N, Palmer S, Mitchell L, Ismail S, Gohlke A, Riley JS, Tait SWG, Gammage P, Soares LL, Macpherson IR, Norman JC
The Journal of cell biology 2021 Dec 6;220(12)

Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization.

Assar EA, Tumbarello DA
Frontiers in cell and developmental biology 2020;8:733

Autophagy promotes growth of tumors with high mutational burden by inhibiting a T-cell immune response.

Poillet-Perez L, Sharp DW, Yang Y, Laddha SV, Ibrahim M, Bommareddy PK, Sherrie Hu Z, Vieth J, Haas M, Bosenberg MW, Rabinowitz JD, Cao J, Guan JL, Ganesan S, Chan CS, Mehnert JM, Lattime EC, White E
Nature cancer 2020 Sep;1(9):923-934

Autophagic adaptation to oxidative stress alters peritoneal residential macrophage survival and ovarian cancer metastasis.

Xia H, Li S, Li X, Wang W, Bian Y, Wei S, Grove S, Wang W, Vatan L, Liu JR, McLean K, Rattan R, Munkarah A, Guan JL, Kryczek I, Zou W
JCI insight 2020 Sep 17;5(18)

Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System.

Simpson LM, Macartney TJ, Nardin A, Fulcher LJ, Röth S, Testa A, Maniaci C, Ciulli A, Ganley IG, Sapkota GP
Cell chemical biology 2020 Sep 17;27(9):1164-1180.e5

Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.

You M, Miao Z, Tian J, Hu F
European journal of nutrition 2020 Oct;59(7):2875-2892

10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells.

You M, Miao Z, Sienkiewicz O, Jiang X, Zhao X, Hu F
International immunopharmacology 2020 Jul;84:106501

Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.

Lindner P, Christensen SB, Nissen P, Møller JV, Engedal N
Cell communication and signaling : CCS 2020 Jan 27;18(1):12

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.

Leidal AM, Huang HH, Marsh T, Solvik T, Zhang D, Ye J, Kai F, Goldsmith J, Liu JY, Huang YH, Monkkonen T, Vlahakis A, Huang EJ, Goodarzi H, Yu L, Wiita AP, Debnath J
Nature cell biology 2020 Feb;22(2):187-199

TBC1D9 regulates TBK1 activation through Ca²⁺ signaling in selective autophagy.

Nozawa T, Sano S, Minowa-Nozawa A, Toh H, Nakajima S, Murase K, Aikawa C, Nakagawa I
Nature communications 2020 Feb 7;11(1):770

The ER-Localized Transmembrane Protein TMEM39A/SUSR2 Regulates Autophagy by Controlling the Trafficking of the PtdIns(4)P Phosphatase SAC1.

Miao G, Zhang Y, Chen D, Zhang H
Molecular cell 2020 Feb 6;77(3):618-632.e5

Bone-derived mesenchymal stem cells alleviate compression-induced apoptosis of nucleus pulposus cells by N6 methyladenosine of autophagy.

Li G, Song Y, Liao Z, Wang K, Luo R, Lu S, Zhao K, Feng X, Liang H, Ma L, Wang B, Ke W, Yin H, Zhan S, Li S, Wu X, Zhang Y, Yang C
Cell death & disease 2020 Feb 6;11(2):103

Aberrant autophagosome formation occurs upon small molecule inhibition of ULK1 kinase activity.

Zachari M, Longo M, Ganley IG
Life science alliance 2020 Dec;3(12)

Inositol Polyphosphate Multikinase Inhibits Liquid-Liquid Phase Separation of TFEB to Negatively Regulate Autophagy Activity.

Chen D, Wang Z, Zhao YG, Zheng H, Zhao H, Liu N, Zhang H
Developmental cell 2020 Dec 7;55(5):588-602.e7

ERdj8 governs the size of autophagosomes during the formation process.

Yamamoto YH, Kasai A, Omori H, Takino T, Sugihara M, Umemoto T, Hamasaki M, Hatta T, Natsume T, Morimoto RI, Arai R, Waguri S, Sato M, Sato K, Bar-Nun S, Yoshimori T, Noda T, Nagata K
The Journal of cell biology 2020 Aug 3;219(8)

ATG5 cancer mutations and alternative mRNA splicing reveal a conjugation switch that regulates ATG12-ATG5-ATG16L1 complex assembly and autophagy.

Wible DJ, Chao HP, Tang DG, Bratton SB
Cell discovery 2019;5:42

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Zachari M, Gudmundsson SR, Li Z, Manifava M, Cugliandolo F, Shah R, Smith M, Stronge J, Karanasios E, Piunti C, Kishi-Itakura C, Vihinen H, Jokitalo E, Guan JL, Buss F, Smith AM, Walker SA, Eskelinen EL, Ktistakis NT
Developmental cell 2019 Sep 9;50(5):627-643.e5

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Yuan F, Jin X, Li D, Song Y, Zhang N, Yang X, Wang L, Zhu WG, Tian C, Zhao Y
Nucleic acids research 2019 Sep 5;47(15):8096-8110

Autophagy Regulates Craniofacial Bone Acquisition.

Thomas N, Choi HK, Wei X, Wang L, Mishina Y, Guan JL, Liu F
Calcified tissue international 2019 Nov;105(5):518-530

SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis.

Lan Y, Sullivan PM, Hu F
Autophagy 2019 May;15(5):871-885

The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.

Ho WY, Tai YK, Chang JC, Liang J, Tyan SH, Chen S, Guan JL, Zhou H, Shen HM, Koo E, Ling SC
Autophagy 2019 May;15(5):827-842

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Chino H, Hatta T, Natsume T, Mizushima N
Molecular cell 2019 Jun 6;74(5):909-921.e6

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Huang X, Gan G, Wang X, Xu T, Xie W
Autophagy 2019 Jul;15(7):1258-1279

Iron Supply via NCOA4-Mediated Ferritin Degradation Maintains Mitochondrial Functions.

Fujimaki M, Furuya N, Saiki S, Amo T, Imamichi Y, Hattori N
Molecular and cellular biology 2019 Jul 15;39(14)

Hyperosmotic Stress Induces Unconventional Autophagy Independent of the Ulk1 Complex.

Tamura N, Kageyama S, Komatsu M, Waguri S
Molecular and cellular biology 2019 Aug 15;39(16)

Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation.

Kumar S, Gu Y, Abudu YP, Bruun JA, Jain A, Farzam F, Mudd M, Anonsen JH, Rusten TE, Kasof G, Ktistakis N, Lidke KA, Johansen T, Deretic V
Developmental cell 2019 Apr 8;49(1):130-144.e6

Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.

Vargas JNS, Wang C, Bunker E, Hao L, Maric D, Schiavo G, Randow F, Youle RJ
Molecular cell 2019 Apr 18;74(2):347-362.e6

Systematic analysis of ATG13 domain requirements for autophagy induction.

Wallot-Hieke N, Verma N, Schlütermann D, Berleth N, Deitersen J, Böhler P, Stuhldreier F, Wu W, Seggewiß S, Peter C, Gohlke H, Mizushima N, Stork B
Autophagy 2018;14(5):743-763

RB1CC1-enhanced autophagy facilitates PSCs activation and pancreatic fibrogenesis in chronic pancreatitis.

Li L, Wang G, Hu JS, Zhang GQ, Chen HZ, Yuan Y, Li YL, Lv XJ, Tian FY, Pan SH, Bai XW, Sun B
Cell death & disease 2018 Sep 20;9(10):952

Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation.

Morita K, Hama Y, Izume T, Tamura N, Ueno T, Yamashita Y, Sakamaki Y, Mimura K, Morishita H, Shihoya W, Nureki O, Mano H, Mizushima N
The Journal of cell biology 2018 Nov 5;217(11):3817-3828

Comparison of the expression of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α in oral squamous cell carcinoma and lymph node metastases of humans and mice.

Guo M, Mu Y, Yu D, Li J, Chen F, Wei B, Bi S, Yu J, Liang F
Oncology letters 2018 Feb;15(2):1639-1645

Galectin-8-mediated selective autophagy protects against seeded tau aggregation.

Falcon B, Noad J, McMahon H, Randow F, Goedert M
The Journal of biological chemistry 2018 Feb 16;293(7):2438-2451

Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.

Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N
The Journal of cell biology 2018 Aug 6;217(8):2633-2645

Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy.

Wang C, Wang H, Zhang D, Luo W, Liu R, Xu D, Diao L, Liao L, Liu Z
Nature communications 2018 Aug 28;9(1):3492

The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.

Zhao YG, Liu N, Miao G, Chen Y, Zhao H, Zhang H
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Muniz-Feliciano L, Doggett TA, Zhou Z, Ferguson TA
Autophagy 2017;13(12):2072-2085

Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis.

Ge L, Zhang M, Kenny SJ, Liu D, Maeda M, Saito K, Mathur A, Xu K, Schekman R
EMBO reports 2017 Sep;18(9):1586-1603

Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine.

Chen X, Clark J, Wunderlich M, Fan C, Davis A, Chen S, Guan JL, Mulloy JC, Kumar A, Zheng Y
Autophagy 2017 May 4;13(5):955-966

Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection.

Pandey A, Ding SL, Qin QM, Gupta R, Gomez G, Lin F, Feng X, Fachini da Costa L, Chaki SP, Katepalli M, Case ED, van Schaik EJ, Sidiq T, Khalaf O, Arenas A, Kobayashi KS, Samuel JE, Rivera GM, Alaniz RC, Sze SH, Qian X, Brown WJ, Rice-Ficht A, Russell WK, Ficht TA, de Figueiredo P
Cell host & microbe 2017 May 10;21(5):637-649.e6

Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.

Nishimura T, Tamura N, Kono N, Shimanaka Y, Arai H, Yamamoto H, Mizushima N
The EMBO journal 2017 Jun 14;36(12):1719-1735

Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.

Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch AK, Müller S, Dikic I, Mittelbronn M, Behrends C
eLife 2017 Feb 14;6

Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets.

Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, Mizushima N
FEBS letters 2017 Dec;591(23):3819-3830

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Sasai M, Sakaguchi N, Ma JS, Nakamura S, Kawabata T, Bando H, Lee Y, Saitoh T, Akira S, Iwasaki A, Standley DM, Yoshimori T, Yamamoto M
Nature immunology 2017 Aug;18(8):899-910

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications 2016 May 18;4(1):51

Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide.

Wang C, Chen S, Yeo S, Karsli-Uzunbas G, White E, Mizushima N, Virgin HW, Guan JL
The Journal of cell biology 2016 Feb 29;212(5):545-60

Distinct roles of autophagy-dependent and -independent functions of FIP200 revealed by generation and analysis of a mutant knock-in mouse model.

Chen S, Wang C, Yeo S, Liang CC, Okamoto T, Sun S, Wen J, Guan JL
Genes & development 2016 Apr 1;30(7):856-69

Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium.

Yao J, Jia L, Khan N, Lin C, Mitter SK, Boulton ME, Dunaief JL, Klionsky DJ, Guan JL, Thompson DA, Zacks DN
Autophagy 2015;11(6):939-53

Macroautophagic cargo sequestration assays.

Seglen PO, Luhr M, Mills IG, Sætre F, Szalai P, Engedal N
Methods (San Diego, Calif.) 2015 Mar;75:25-36

MiR224-3p inhibits hypoxia-induced autophagy by targeting autophagy-related genes in human glioblastoma cells.

Guo X, Xue H, Guo X, Gao X, Xu S, Yan S, Han X, Li T, Shen J, Li G
Oncotarget 2015 Dec 8;6(39):41620-37

Imaging autophagy.

Karanasios E, Stapleton E, Manifava M, Ktistakis NT
Current protocols in cytometry 2014 Jul 1;69:12.34.1-12.34.16

Suppression of autophagy by FIP200 deletion leads to osteopenia in mice through the inhibition of osteoblast terminal differentiation.

Liu F, Fang F, Yuan H, Yang D, Chen Y, Williams L, Goldstein SA, Krebsbach PH, Guan JL
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2013 Nov;28(11):2414-30

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP(RB1CC1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line