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LearnPA5-69698
antibody from Invitrogen Antibodies
Targeting: RB1CC1
ATG17, Cc1, DRAGOU14, FIP200, KIAA0203, PPP1R131
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-69698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FIP200 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references ZBTB28 induces autophagy by regulation of FIP200 and Bcl-XL facilitating cervical cancer cell apoptosis.
Li L, Gong Y, Xu K, Chen W, Xia J, Cheng Z, Li L, Yu R, Mu J, Le X, Xiang Q, Peng W, Tang J, Xiang T
Journal of experimental & clinical cancer research : CR 2021 Apr 30;40(1):150
Journal of experimental & clinical cancer research : CR 2021 Apr 30;40(1):150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry was performed on 293T cells. Sample was probed with a FIP200 polyclonal antibody (Product # PA5-69698) with a dilution of 0.2-1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 ZBTB28 upregulated FIP200 to induce autophagy and apoptosis. a qRT-PCR results of FIP200 mRNA expression in stable transfected cervical cancer cells. b Structure of wild-type and mutant FIP200 reporter plasmid. c % Input of FIP200 DNA by HA-tag antibody were tested by ChIP-PCR. Then products of ChIP-PCR were used for electrophoresis. d The effect of ZBTB28 on FIP200 promoter activity was detected by dual luciferase reporter system. e CaSki and HeLa cells were transfected with pcDNA3.1 or ZBTB28, then tested promoter luciferase activity of mutant FIP200 in live cells. f Forty eight hours after transfection with siFIP200 or control siRNA, the expression of FIP200, p62, LC3, Cleaved-casp8, Casp3, Cleaved-casp3 and Cleaved-PARP were evaluated by western blot. beta-actin was used as negative control. g Stable transfected CaSki and HeLa cells were re-transfected with siFIP200 or control siRNA. Seventy two hours after transfection, cell viability was assessed by CCK8 assay. * p < 0. 05, ** p < 0. 01, *** p < 0. 001
