TA504279

antibody from OriGene

Targeting: BLOC1S6 HPS9, PA, PLDN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504279

Provider

OriGene

Proper citation

OriGene Cat#TA504279, RRID:AB_11125259

Product name

PLDN (Pallidin) mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Antibody type

Monoclonal

Description

PLDN (Pallidin) mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Host

Mouse

Conjugate

Unconjugated

Epitope

BLOC1S6

Isotype

IgG

Antibody clone number

OTI1H9

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (RC201514, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PLDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504279)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201514 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLDN antibody(TA504279), and then analyzed by flow cytometry.

Validation comment

FC