LS-C675425

antibody from LSBio

Targeting: BLOC1S6 HPS9, PA, PLDN

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C675425

Provider

LSBio

Product name

PLDN / Pallidin Antibody LS-C675425

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes: BLOC1S6 antibody at 3.33 ug/ml Lane 1: A431 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20,9 kDa Observed band size: 20 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating BLOC1S6 in HEK293 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HEK293 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HEK293 whole cell lysate(500ug) Lane 3: HEK293 whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating BLOC1S6 in HEK293 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HEK293 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HEK293 whole cell lysate(500ug) Lane 3: HEK293 whole cell lysate (20ug)