16457-1-AP

antibody from Proteintech Group

Targeting: MRPS35 MDS023, MRPS28

Provider product page for 16457-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [27]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 16457-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16457-1-AP, RRID:AB_2146521
Product name: MRPS35 antibody
Antibody type: Polyclonal
Description: MRPS35 antibody (Cat. #16457-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MRPS35 protein structure - 16457-1-AP shown in red.

Submitted references Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA.

Ng KY, Battersby BJ
Methods in molecular biology (Clifton, N.J.) 2023;2661:101-117

A STAT3 protein complex required for mitochondrial mRNA stability and cancer.

Fernando CD, Jayasekara WSN, Inampudi C, Kohonen-Corish MRJ, Cooper WA, Beilharz TH, Josephs TM, Garama DJ, Gough DJ
Cell reports 2023 Sep 26;42(9):113033

Autoregulatory control of mitochondrial glutathione homeostasis.

Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K
Science (New York, N.Y.) 2023 Nov 17;382(6672):820-828

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals.

Kenny TC, Khan A, Son Y, Yue L, Heissel S, Sharma A, Pasolli HA, Liu Y, Gamazon ER, Alwaseem H, Hite RK, Birsoy K
Cell metabolism 2023 Jun 6;35(6):1057-1071.e12

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.

Amarasekera SSC, Hock DH, Lake NJ, Calvo SE, Grønborg SW, Krzesinski EI, Amor DJ, Fahey MC, Simons C, Wibrand F, Mootha VK, Lek M, Lunke S, Stark Z, Østergaard E, Christodoulou J, Thorburn DR, Stroud DA, Compton AG
Human molecular genetics 2023 Jul 20;32(15):2441-2454

Nonstop mRNAs generate a ground state of mitochondrial gene expression noise.

Ng KY, Lutfullahoglu Bal G, Richter U, Safronov O, Paulin L, Dunn CD, Paavilainen VO, Richer J, Newman WG, Taylor RW, Battersby BJ
Science advances 2022 Nov 16;8(46):eabq5234

Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations.

Molina-Berenguer M, Vila-Julià F, Pérez-Ramos S, Salcedo-Allende MT, Cámara Y, Torres-Torronteras J, Martí R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Jan;36(1):e22091

circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma.

Li P, Song R, Yin F, Liu M, Liu H, Ma S, Jia X, Lu X, Zhong Y, Yu L, Li X, Li X
Molecular therapy : the journal of the American Society of Gene Therapy 2022 Jan 5;30(1):431-447

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism.

Shu Y, Hao Y, Feng J, Liu H, Li ST, Feng J, Jiang Z, Ye L, Zhou Y, Sun Y, Zhou Z, Wei H, Gao P, Zhang H, Sun L
The EMBO journal 2022 Dec 1;41(23):e111550

Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.

Ruzzenente B, Metodiev MD
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60

Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.

Lee RG, Gao J, Siira SJ, Shearwood AM, Ermer JA, Hofferek V, Mathews JC, Zheng M, Reid GE, Rackham O, Filipovska A
Journal of cell science 2020 Jul 24;133(14)

TRMT2B is responsible for both tRNA and rRNA m(5)U-methylation in human mitochondria.

Powell CA, Minczuk M
RNA biology 2020 Apr;17(4):451-462

RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Reyes A, Rusecka J, Tońska K, Zeviani M
Frontiers in genetics 2019;10:1393

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schöndorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, Larsson NG
Cell reports 2019 Nov 5;29(6):1728-1738.e9

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Rudler DL, Hughes LA, Perks KL, Richman TR, Kuznetsova I, Ermer JA, Abudulai LN, Shearwood AJ, Viola HM, Hool LC, Siira SJ, Rackham O, Filipovska A
Science advances 2019 Dec;5(12):eaay2118

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Ferreira N, Perks KL, Rossetti G, Rudler DL, Hughes LA, Ermer JA, Scott LH, Kuznetsova I, Richman TR, Narayana VK, Abudulai LN, Shearwood AJ, Cserne Szappanos H, Tull D, Yeoh GC, Hool LC, Filipovska A, Rackham O
The EMBO journal 2019 Dec 16;38(24):e102155

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

Siira SJ, Rossetti G, Richman TR, Perks K, Ermer JA, Kuznetsova I, Hughes L, Shearwood AJ, Viola HM, Hool LC, Rackham O, Filipovska A
EMBO reports 2018 Oct;19(10)

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals.

Kühl I, Miranda M, Atanassov I, Kuznetsova I, Hinze Y, Mourier A, Filipovska A, Larsson NG
eLife 2017 Nov 14;6

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Perks KL, Ferreira N, Richman TR, Ermer JA, Kuznetsova I, Shearwood AJ, Lee RG, Viola HM, Johnstone VPA, Matthews V, Hool LC, Rackham O, Filipovska A
Science advances 2017 Aug;3(8):e1700677

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

Lake NJ, Webb BD, Stroud DA, Richman TR, Ruzzenente B, Compton AG, Mountford HS, Pulman J, Zangarelli C, Rio M, Boddaert N, Assouline Z, Sherpa MD, Schadt EE, Houten SM, Byrnes J, McCormick EM, Zolkipli-Cunningham Z, Haude K, Zhang Z, Retterer K, Bai R, Calvo SE, Mootha VK, Christodoulou J, Rötig A, Filipovska A, Cristian I, Falk MJ, Metodiev MD, Thorburn DR
American journal of human genetics 2017 Aug 3;101(2):239-254

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Richman TR, Spåhr H, Ermer JA, Davies SM, Viola HM, Bates KA, Papadimitriou J, Hool LC, Rodger J, Larsson NG, Rackham O, Filipovska A
Nature communications 2016 Jun 20;7:11884

CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.

Szczepanowska K, Maiti P, Kukat A, Hofsetz E, Nolte H, Senft K, Becker C, Ruzzenente B, Hornig-Do HT, Wibom R, Wiesner RJ, Krüger M, Trifunovic A
The EMBO journal 2016 Dec 1;35(23):2566-2583

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Rackham O, Busch JD, Matic S, Siira SJ, Kuznetsova I, Atanassov I, Ermer JA, Shearwood AM, Richman TR, Stewart JB, Mourier A, Milenkovic D, Larsson NG, Filipovska A
Cell reports 2016 Aug 16;16(7):1874-90

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

Richman TR, Ermer JA, Davies SM, Perks KL, Viola HM, Shearwood AM, Hool LC, Rackham O, Filipovska A
PLoS genetics 2015 Mar;11(3):e1005089

SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.

Lagouge M, Mourier A, Lee HJ, Spåhr H, Wai T, Kukat C, Silva Ramos E, Motori E, Busch JD, Siira S, German Mouse Clinic Consortium, Kremmer E, Filipovska A, Larsson NG
PLoS genetics 2015 Aug;11(8):e1005423

Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.

Dogan SA, Pujol C, Maiti P, Kukat A, Wang S, Hermans S, Senft K, Wibom R, Rugarli EI, Trifunovic A
Cell metabolism 2014 Mar 4;19(3):458-69

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

Almajan ER, Richter R, Paeger L, Martinelli P, Barth E, Decker T, Larsson NG, Kloppenburg P, Langer T, Rugarli EI
The Journal of clinical investigation 2012 Nov;122(11):4048-58

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP(MRPS35 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using MRPS35 antibody 16457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line